Abstract The theory and several circuits for supercharging is described for single micro and patch electrode voltage and current clamping. Publication types Research Support, Non-U. Gov't Research Support, U. Gov't, P. Please contact us for further information and prices. Related products. Show Details. WD — Window Discriminator. Subscribe to our Newsletter Email address:. Recent News Attention Neuroimagers! ALA Scientific Instruments is officially opened!
Search Site. Accept settings Hide notification only Settings. Cookie and Privacy Settings. How we use cookies. Essential Website Cookies. Check to enable permanent hiding of message bar and refuse all cookies if you do not opt in. We need 2 cookies to store this setting. Otherwise you will be prompted again when opening a new browser window or new a tab. Other external services. Accept settings Hide notification only. Turns off current injection and cap. SEC no oscillation shut-off. Overcompensation of cap.
No cell penetration unit SEC modular penetration unit available. Switching frequency: linear control 1. Electrode potential: max. Sensitivity: 0. SEC unfiltered or 5 kHz, internally adjustable. If cells in acute slices are investigated, an infrared DIC is recommendable to visualize the membrane. The microscope should be placed on an anti-vibration table because any movement could be fatal to the seal between the pipette and the membrane. A micromanipulator is needed to move the pipette precisely.
Very fine pipettes are formed by heating and pulling small glass or quartz capillary tubes. The tip of the pipette is heat-polished in a microforge to gain a high-resistance seal onto the membrane. The pipette is filled with a solution that resembles either the extracellular solution or the cytoplasm, depending on the recording mode.
The pipette is mounted on a micromanipulator to permit precise movements towards the cell membrane. For conductance of the current a chlorided silver wire is used. The bath electrode, which is also a chlorided silver wire, sets zero current value. A differential amplifier with a low-noise transistor is connected to a computer for data acquisition and digitization. Specific software can be purchased to control the amplifier and analyze the data.
An oscilloscope can alternatively be used to monitor the currents. If desired, a perfusion system can be added to the setup. Substances can either be applied via a perfusion pencil or by using a POC perfusion open and closed chamber. Patch-clamp experiments are used to approach a huge variety of physiological questions, not only in neuroscience.
During the last two decades patch-clamp recordings have also become more important for the investigation of ion channels in non-excitable cells. It is also a very important method in medical research, since many diseases are related to a malfunction of definite ion channels. In pharmacological research, automated patch-clamping is used to screen potent substances for ion channel modifications.
The membrane current and changes in fluorescence are recorded simultaneously. Similar experiments can be performed with pH- or Cl - -sensitive dyes.
The video shows the preparation of mouse brain slices using a vibrating microtome. It depicts the whole workflow beginning with the dissection using a stereo microscope, the embedding in low gelling temperature agarose and the slicing itself. In this video the workflow for performing electrophysiological measurements on acute brain slices using a fixed stage microscope. The video shows an exemplary workflow for performing electrophysiology on fluorescent cultured cells using an inverted microscope.
It starts with the preparation of the patch pipettes and ends with the actual measurement on the microscope. Talk to our experts. We are happy to answer all your questions and concerns.
Do you prefer personal consulting? You will find a more detailed list of local contacts here. November 09, Tutorial. A glass pipette containing electrolyte solution is tightly sealed onto the cell membrane and thus isolates a membrane patch electrically.
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